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Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
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Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

DOI:
10.3791/65169-v

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06:21 min

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April 07, 2023

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,
1Department of Medicine, Division of Endocrinology, Diabetes and Metabolism,University of Rochester School of Medicine and Dentistry

Chapters

  • 00:04Introduction
  • 01:02Surgery to Implant Osmotic Minipump
  • 02:59Cerebrospinal Fluid (CSF) Collection Procedure
  • 05:11Results: Glucose Infusion in CSF Using the Osmotic Minipump
  • 05:31Conclusion

Summary

자동 번역

자동 번역

This article describes a detailed protocol to increase glucose concentration in the cerebrospinal fluid (CSF) of mice. This approach can be useful for studying the effects of high CSF glucose on neurodegeneration, cognition, and peripheral glucose metabolism in mice.

Tags

Osmotic Minipump ImplantationCSF Glucose LevelsMouse BrainCognitive DysfunctionNeurodegenerative DisordersStereotaxic SurgeryBregmaDura MaterCannulaDental Cement

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