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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

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测量哺乳动物线粒体功能的氧非依赖性测定

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JoVE 신문 생화학

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

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测量哺乳动物线粒体功能的氧非依赖性测定

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

DOI:

10.3791/65184-v

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01:14 min

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May 19, 2023

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Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli²

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Tags

Mitochondrial Function Oxygen-independent Assays Hypoxia Metabolite Analysis Fumarate Electron Transport Chain ATP Production Pyrimidine Biosynthesis NADH Oxidation Superoxide Production Respirometry

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