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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

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哺乳類のミトコンドリア機能を測定するための酸素非依存性アッセイ(英語)

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Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

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哺乳類のミトコンドリア機能を測定するための酸素非依存性アッセイ(英語)

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

DOI:

10.3791/65184-v

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01:14 min

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May 19, 2023

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Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli²

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

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Keywords: Mitochondrial Function Oxygen-independent Assays Hypoxia Metabolite Analysis Fumarate Electron Transport Chain ATP Production Pyrimidine Biosynthesis NADH Oxidation Superoxide Production Respirometry

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哺乳類のミトコンドリア機能を測定するための酸素非依存性アッセイ(英語)

¹³名C₄-アスパラギン酸および ¹³C₅-グルタミン安定同位体トレーシング、LC-MS分析、およびSDH活性測定

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