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Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis

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一种用于FACS分离的小鼠卫星细胞的CUT&RUN分析的有效方案

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An Efficient Protocol for CUT&RUN Analysis of FACS-Isolated Mouse Satellite Cells

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一种用于FACS分离的小鼠卫星细胞的CUT&RUN分析的有效方案

Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis

DOI:

10.3791/65215-v

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02:40 min

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July 07, 2023

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Kamar Ghaibour*¹, Joe Rizk*¹, Claudine Ebel, Tao Ye, Muriel Philipps, Valérie Schreiber, Daniel Metzger, Delphine Duteil

¹CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258,Université de Strasbourg

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Keywords: CUT&RUN FACS Mouse Satellite Cells Nuclear Receptors Secosteroid Steroid Receptor Signaling Skeletal Muscle Androgen Receptor Cistrome Analysis Chromatin Landscape Genome-wide Analysis Transcription Factor Recruitment Cell Isolation Cell Sorting

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