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면역학 및 감염병학
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Staining and Imaging to Visualize Neutrophil Extracellular Traps
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Immunfluoreszenz-Bildgebung von neutrophilen extrazellulären Fallen in menschlichen und Mausgeweben
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues
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Immunfluoreszenz-Bildgebung von neutrophilen extrazellulären Fallen in menschlichen und Mausgeweben

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:
10.3791/65272-v

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01:53 min

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August 18, 2023

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, , , , ,
1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, 2Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, 3Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Keywords: Immunofluorescence ImagingNeutrophil Extracellular TrapsNETsAntibody SelectionEpitope MaskingAutofluorescenceStandardized ProtocolAntigen RetrievalPermeabilizationCitrullinated Histone 3Autofluorescence-reducing AgentElectron MicroscopyAutoimmune DiseasesSepsisInflammation

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