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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Generatie en manipulatie van darmorganoïden van ratten

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Generation and Manipulation of Rat Intestinal Organoids

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Generatie en manipulatie van darmorganoïden van ratten

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/65343-v

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01:58 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Keywords: Rat Intestinal Organoids Genetic Manipulation Lentiviral Infection Transient Transfection Intestinal Biology Mouse Intestinal Organoids Human Intestinal Organoids Physiological Relevance Disease Modeling Drug Discovery Personalized Treatment Stem Cell Dynamics Cell Fate Decisions

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