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经瞳孔引导下视网膜下移植物的视网膜下移植物经巩膜移植视网膜变性小鼠模型

JoVE 신문
신경과학
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JoVE 신문 신경과학

Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

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经瞳孔引导下视网膜下移植物的视网膜下移植物经巩膜移植视网膜变性小鼠模型

DOI:

10.3791/65448-v

•

07:37 min

•

January 26, 2024

•

Ying V. Liu, Kang V. Li, Zhuolin Li, Yuchen Lu, Minda M. McNally, Edward P. Esposito, Kanza Aziz, Mandeep S. Singh²

¹Wilmer Eye Institute,Johns Hopkins University School of Medicine, ²Department of Genetic Medicine,Johns Hopkins University School of Medicine

Chapters

00:00Introduction
00:31Animals
00:58Collect Neural Retina
01:58Prepare Donor Retinal Suspension
02:19Prepare Donor Retinal Sheets
02:36Prepare Mouse Recipients
03:46Subretinal Transplantation of Retinal Grafts
05:12Post-surgery Administration
05:48Results
07:04Conclusion

Summary

자동 번역

该协议提出了一种经瞳孔视觉引导的经巩膜方法，可在有或没有视网膜变性的小鼠接受者中安全准确地输送视网膜下细胞移植物，手术并发症发生率低。

Tags

Keywords: Transpupillary-guided Trans-scleral Subretinal Grafts Retinal Degeneration Mouse Model EGFP OPN1LW-EGFP/NRL Rd1/NS Papain Retinal Sheet Anesthesia Pupil Dilation Transpupillary Visualization

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