/

/

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

/

יצירת Protein-E CENP-E-^/- Knockout Cell Lines באמצעות מערכת CRISPR/Cas9

JoVE 신문
생물학
Author Produced

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Generation of Centromere-Associated Protein-E CENP-E^-/- Knockout Cell Lines using the CRISPR/Cas9 System

Please note that all translations are automatically generated. Click here for the English version.

יצירת Protein-E CENP-E-^/- Knockout Cell Lines באמצעות מערכת CRISPR/Cas9

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/65476-v

•

02:19 min

•

June 23, 2023

•

Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

Keywords: CENP-E CRISPR/Cas9 Knockout HeLa Cells Chromosome Alignment Mitotic Defects Cell Cycle Arrest Cell Death Kinetochore-microtubule Capture Spindle Assembly Checkpoint

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

יצירת Protein-E CENP-E-^/- Knockout Cell Lines באמצעות מערכת CRISPR/Cas9

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Read Article