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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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使用荧光成像快速体外细胞毒性评估表达嵌合抗原受体的 Jurkat

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Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

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使用荧光成像快速体外细胞毒性评估表达嵌合抗原受体的 Jurkat

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

DOI:

10.3791/65560-v

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01:44 min

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October 27, 2023

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs, Jacqueline Babai, Riza Alker, Andrew K. Godwin^5,6, David Akhavan^2,3

¹Department of Radiation Oncology,University of Kansas Cancer Center, ²Department of Cancer Biology,University of Kansas Cancer Center, ³BioEngineering Program,University of Kansas, ⁴Department of Pathology and Laboratory Medicine,University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine,University of Kansas Medical Center

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Keywords: CAR T-cells Jurkat Cells Cytotoxicity Fluorescent Imaging High-throughput Screening CAR Construct Optimization Cancer Immunotherapy

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