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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

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一种简单、快速、部分自动化的方案，用于从冷冻哺乳动物组织中分离单细胞核，用于单细胞核测序

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A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

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一种简单、快速、部分自动化的方案，用于从冷冻哺乳动物组织中分离单细胞核，用于单细胞核测序

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

DOI:

10.3791/65611-v

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01:58 min

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July 28, 2023

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Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Keywords: Single Nuclei Isolation Single Nucleus Sequencing Automated Nuclei Extraction Standardized Protocols Drug Discovery Transcriptomics Gene Expression Robotic Dissociation Chemical Gradient Filtration Automated Cell Counting Mouse Brain Rat Kidney Cynomolgus Liver And Spleen

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