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Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

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用于评估蛋白水解靶向嵌合体（PROTACS）诱导的三元复合物形成的生物物理测定方法的发展和应用

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The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

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用于评估蛋白水解靶向嵌合体（PROTACS）诱导的三元复合物形成的生物物理测定方法的发展和应用

Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

DOI:

10.3791/65718-v

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01:09 min

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January 12, 2024

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Wei Jiang, Holly Soutter

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

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Keywords: Biophysical Assays Ternary Complex Formation Proteolysis Targeting Chimeras (PROTACs)Surface Plasmon Resonance (SPR)Biolayer Interferometry (BLI)Isothermal Titration Calorimetry (ITC)Brd4BD2 VHL PPM1D Cereblon (CRBN)Kinetics Thermodynamics Cooperativity Hook Effect

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