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Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen

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Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing

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Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing

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Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing

Isolation of Nuclei for Multiome Sequencing in Solid Tumor Specimen

DOI:

10.3791/65831-v

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01:39 min

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October 13, 2023

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Deshka S. Foster, Michelle Griffin, Michael Januszyk, Daniel Delitto, Jeffrey A. Norton, Michael T. Longaker

¹Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery,Stanford University

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Keywords: Nuclei Isolation Solid Tumor Multiome Sequencing 10x Genomics Pancreatic Ductal Adenocarcinoma Cryopreservation Nuclear Isolation Cell Heterogeneity Desmoplastic Matrix Cell Yield Cell Quality

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