JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:12Introduction
- 01:07Setting up the JIMT-1 Enhanced Fluorescent Protein (EGFP) Spheroid Model
- 03:43Coating the HCS Plate
- 04:33Transfer of Spheroids to the HCS Plate
- 04:46Pre-treatment of JIMT-1-EGFP Spheroids with Test Compounds
- 05:14Induction of Antibody-Dependent Cellular Cytotoxicity (ADCC) by Adding the Effector Cells
- 07:10Annexin V-647 Staining of Spheroids
- 07:34Imaging
- 11:43Representative Results
- 12:52Conclusion
Hier presenteren we een methode om verbindingen te identificeren die het ADCC-mechanisme moduleren, een belangrijk kankerceldodend mechanisme van antitumorantilichamen. Het cytotoxische effect van NK-cellen wordt gemeten in sferoïden van borstkankercellen in aanwezigheid van Trastuzumab. Beeldanalyse identificeert levende en dode killer- en doelcellen in sferoïden.
