/

/

Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

/

Isolamento di vettori virali adeno-associati attraverso un protocollo cromatografico di affinità con eparina a passaggio singolo e semiautomatico

JoVE 신문
생체공학

This content is Free Access.

JoVE 신문 생체공학

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Please note that all translations are automatically generated. Click here for the English version.

Isolamento di vettori virali adeno-associati attraverso un protocollo cromatografico di affinità con eparina a passaggio singolo e semiautomatico

Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

DOI:

10.3791/66550-v

•

01:57 min

•

April 05, 2024

•

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC – Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC – Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra

Tags

Keywords: Adeno-associated Viral Vectors AAV Purification Heparin Affinity Chromatography Transient Transfection Density Gradient Ultracentrifugation Scalable Production Semi-automated Protocol Mosaic AAV Preclinical Studies

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Isolamento di vettori virali adeno-associati attraverso un protocollo cromatografico di affinità con eparina a passaggio singolo e semiautomatico

Produzione di AAV ricombinante mediante trasfezione transitoria

Purificazione e caratterizzazione FPLC di AAV ricombinante

Read Article