JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:52Introduction
- 02:13Fabrication and Loading of Micropipettes
- 04:02Single-Cell Electroporation Technique
- 09:16Screening for Successfully Electroporated Cells
- 10:16In Vivo 2-Photon Imaging of Neurons Fluorescently Labeled by Single-Cell Electroporation
- 12:46Conclusion
Tags
Single-cell ElectroporationIn VivoIntact Developing BrainDNA DeliveryMacromoleculesIndividual CellsExperimental ManipulationsCell-autonomous EffectsGlobal TreatmentsIn Vivo Imaging TechniquesFluorescent MarkersCellular MorphologyCell GrowthIntracellular EventsXenopus Laevis TadpolesVideo ArticleProcedure For SCEFluorescent DyePlasmid DNANeuronsAlbino Xenopus TadpoleYield OptimizationTwo-photon Fluorescence Imaging
