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Trypsinizing and Subculturing Mammalian Cells
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Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

DOI:
10.3791/755-v

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05:59 min

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June 12, 2008

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,
1Molecular Pathology Laboratory Network, Inc

Chapters

  • 00:00Title
  • 00:37Introduction
  • 00:56Trypsinizig and Subculturing Mammalian cells from a Monolayer
  • 04:00Passaging Cells in Suspension Culture
  • 05:36Conclusion

Summary

자동 번역

자동 번역

As cells reach confluency, they must be subcultured or passaged. This video will demonstrate a procedure for subculturing both adherent and suspension cells.

Tags

TrypsinizingSubculturingMammalian CellsConfluencyPassagingMitotic IndexCell DeathDetachmentPrimary Culture VesselTrypsinizationMechanical MeansCell SuspensionReseedingFresh CulturesSecondary CulturesGrowthFeedingTertiary CulturesTime IntervalCell LineGrowth Rate

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