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免疫印迹分析
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Immunoblot Analysis
DOI:

16:01 min

June 20, 2008

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Chapters

  • 00:00Title/ Prologue
  • 00:56Introduction
  • 01:22Separating Protein Samples by Gel Electrophoresis
  • 02:06Assembling the Immunoblot Sandwich
  • 05:43Transferring Proteins from Gel to Membrane
  • 07:52Immunoprobing Membrane with Directly Conjugated Secondary Antibody
  • 10:45Immunoprobing with Avidin-Biotin Coupling to Secondary Antibody
  • 14:26Visualization of Immunoblot with Chromogenic Substrates
  • 15:32Conclusion

Summary

자동 번역

免疫印迹法(Western印迹)是一个快速,灵敏的蛋白质,利用固有的特异性抗原抗体识别检测和表征的检测。这个视频提供蛋白质分离协议,印迹到膜蛋白,immunoprobing,和可视化,使用显色或化学发光基板。

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