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Chapters
- 00:05Title
- 00:08Introduction
- 01:04Harvest and Processing Of Bone Marrow
- 03:41Culture of Dendritic Cells
- 05:07Sample DC Microscropy and Flow Control
- 06:12Conclusions
Deze video toont de procedure voor de differentiatie van myeloïde dendritische cellen van de muis beenmerg. Isolatie van de muis scheenbeen en dijbeen, en verwerking van het beenmerg worden aangetoond. Foto's tonen celmorfologie voor en na de differentiatie, en figuren afbeelden cel fenotype en IL-12 productie na rijping met behulp van CpG worden weergegeven.
Tags
Myeloid Dendritic CellsBone Marrow PrecursorsImmune MechanismsAntigen Presenting CellsVaccine VectorTibias And FemursIn Vitro Differentiation Of DCsFunctional Activity Of DCsCell Surface MarkersCytokine ProductionMaturation Of DCsFlow Cytometric ComparisonsCo-stimulatory MoleculesIL-12 CytokineCpG StimulationMock TreatmentVaccine Vector ManipulationAntigen-specific Immune ResponsesIn Vitro PulsingRecombinant Viral Vectors
