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Chapters
- 00:00Title
- 00:10Preparations for the Dissection
- 02:40Dissection of the Eye
- 09:42Moving Slices to the Recording Chamber
- 12:08Preparation of patch Electrodes
- 13:21The Recording Rig
- 14:09Whole-cell Recording
- 17:40Recording Light-evoked Currents
- 18:55Discussion/ Conclusion
Cette vidéo montre le processus de la cellule entière enregistrements pince de tension dans la tranche la rétine de la salamandre aquatique tigre. Nous démontrons la préparation de la tranche ainsi que la façon d'effectuer des enregistrements de patch-clamp pendant la stimulation visuelle de la rétine.
Tags
Whole-cell RecordingsLight Evoked Excitatory Synaptic CurrentsRetinal SliceWhole-cell Patch Clamp TechniqueVisual Information ProcessingRetinaAquatic Tiger SalamanderAnimal ModelEye DissectionElectrophysiological RecordingsRecording ChamberWhole-cell Voltage Clamp RecordingsVisual StimuliPhotoreceptorsLight-evoked Current ResponsesPharmacological Agents8-channel Perfusion SystemRetinal Slice PreparationPatch Clamp RecordingsAmacrine CellsBipolar Cells
