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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

DOI:
10.3791/773-v

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13:13 min

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July 09, 2008

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, , ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour,University of California, Irvine (UCI)

Chapters

  • 00:05Title
  • 01:42Bone marrow isolation and resuspension: Removing the femur
  • 03:32Removal and re-suspension of the bone marrow.
  • 06:21Incucyte
  • 07:32Culturing of dendritic cells
  • 11:26Collection of activated, antigen pulsed dendritic cells, intra dermal injection, and 2-photon imaging
  • 12:47Conclusion

Summary

자동 번역

자동 번역

Antigen presentation in secondary lymphoid organs by dendritic cells is crucial for the initiation of the T cell mediated adaptive immune response. Here we demonstrate the culture of bone marrow derived murine dendritic cells, activation, and labeling for 2-photon imaging.

Tags

Bone Marrow DerivedMurine Dendritic Cells2-photon ImagingPreparation MethodCD11c High Dendritic CellsCultureHomingDraining Lymph NodeAntigen PresentationT Cell InteractionEssen Instruments IncucyteDendritic Cell MaturationPeripheral Lymph NodesAntigen-specific InteractionsTwo-photon Imaging

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