JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:37Introduction
- 01:09Explanting the Embryo in Saline
- 01:58Centering the Embryo on a Ring
- 03:09Setting Up the Culture
- 04:07Transferring the Culture to the Incubator
- 05:02Fixing the Embryo
- 07:35Representative Results/Outcome
- 08:17Conclusion
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
