Journal
/
생물학
/
从人死后脑组织神经元细胞核分离
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
Please note that all translations are automatically generated. Click here for the English version.

从人死后脑组织神经元细胞核分离

DOI:
10.3791/914-v

•

10:58 min

•

October 01, 2008

•

,
1Psychiatry, Brudnick Neuropsychiatric Research Institute,University of Massachusetts Medical School

Chapters

  • 00:00Title
  • 00:56Introduction
  • 01:20Extraction of Nuclei from Postmortem Human Brain
  • 03:34Immunostaining of Purified Nuclei
  • 05:54Isolation of Neuronal Nuclei by Fluorescence Activated Sorting
  • 08:50Concentration of Neuronal Nuclei by Centrifugation
  • 10:08Conclusion

Summary

자동 번역

자동 번역

脑组织细胞的异质性，构成一个明显的局限性染色，因为大多数分析缺乏单细胞分辨率的表观遗传标记的研究。通常是神经元与神经胶质细胞和其他非神经元细胞混合。我们提供了一个协议提取和收集从人脑的神经元细胞核。

Tags

Neuronal Nuclei IsolationNeuronal ChromatinNuclear OrganizationChromatin And DNA-based AssaysSingle Cell ResolutionCellular HeterogeneityGlia CellsNon-neuronal CellsCell-type Specific CulturesCNS CellsEpigenetic MechanismsHypotonic Lysis BufferUltracentrifugationImmunotaggingAnti-NeuN AntibodyFluorescence-activated SortingChromatin Immunoprecipitation Studies

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/914?article_type=v

一个人脑组织中的染色质含量
check_url/kr/914?article_type=v

人死后脑组织中的单细胞群获得高品质的RNA
check_url/kr/914?article_type=v

从小鼠和人类结肠组织初级肌成纤维细胞的分离
check_url/kr/914?article_type=v

标记的细胞核从亲和力为基础的隔离<em>果蝇</em>组织的基因表达分析
check_url/kr/914?article_type=v

从小鼠胰腺RNA提取：核糖核酸酶丰富的组织
check_url/kr/914?article_type=v

Y-27632 丰富成人皮肤组织中人类黑色素细胞的产量
check_url/kr/914?article_type=v

使用洗涤剂和酶无酶方法从整个组织进行核分离
check_url/kr/914?article_type=v

使用基于多反应监测的人类脑组织蛋白质的多反应监测工作流程定量蛋白质组学工作流程
check_url/kr/914?article_type=v

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
check_url/kr/914?article_type=v

培养的人间充质干细胞细胞外囊泡的分离、表征和治疗应用

Read Article