JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:29Preparing the lipids
- 02:13Determining how much ICAM-1 and MHC are deposited on the planar bilayer
- 03:22Cleaning the glass cover slips for forming planar bilayers
- 04:47Forming the bilayers
- 08:27Charging the bilayers and introducing His-tagged proteins
- 10:20Introducing primary T cells into the flow cell
- 11:36Conclusion
Tags
Supported Planar BilayersImmunological SynapseKinapseMolecular InteractionsLymphocytesAntigen Presenting CellsNi2+-chelating LipidsRecombinant Cell Adhesion ProteinsMHC ProteinsPoly-histidine TagsGlass SurfacesSpecialized ChamberFlow-cellTIRF MicroscopySynapse FormationT Cell LocomotionReceptor SortingReceptor Degradation
