JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:54Introduction
- 01:24Fixing the Embryos
- 02:39Preparing Embryos for Antibody Treatment
- 04:04Antibody Incubation
- 05:38Color Reaction
- 06:48Embryo Processing for Photography and Histology
- 08:18Representative Results
- 09:30Conclusion
Tags
Whole Mount Antibody StainingChick EmbryoEarly Embryonic DevelopmentAntibody ExpressionDeveloping BrainNeural TubeSomiteHRP Conjugated Secondary AntibodiesParaformaldehyde FixationInactivation Of Endogenous PeroxidasePrimary Antibody ExposureWashesSecondary Antibody IncubationPeroxidase ActivityDiaminobenzidine Substrate ReactionWax SectioningAntigen SitesJane DoddTom Jessell
