A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.
Part 1: Preparation of the CBB staining solution
Part 2: SDS-PAGE
Part 3: Staining of the gel
Part 4: Representative Results:
See Fig. 1 for a properly stained gel following the described procedure.
See Fig. 2 for a gel that has not been washed long enough before staining and residual SDS inhibits efficient staining. Note that the marker lanes (*) contain the same amount of marker proteins.
Fig. 1: Representative gel stained after loading samples of a protein purification (*: molecular weight marker).
Fig. 2: CBB stained gel that has not been washed long enough before CBB staining. The protein bands appear weaker (note that the marker lane * contains the same amount of protein as in Fig. 1).
The authors have nothing to disclose.
We would like to acknowledge the technical assistance of Ines Racké.
Material Name | Tipo | Company | Catalogue Number | Comment |
---|---|---|---|---|
Coomassie Brilliant Blue G-250 | AppliChem | A3480 | any other CBB G-250 could be used as well | |
Concentrated HCl |