Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

Published: April 21, 2023

doi:

10.3791/200140

Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta, Alfonso Valencia³, Biola M. Javierre⁴

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

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Keywords: DNA Library Scarce Cell DNA Biotin Pull-down PCR Amplification Library Capture Streptavidin Beads Binding Buffer Wash Buffer DNA Purification Paramagnetic Beads Automated Electrophoresis

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Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library. J. Vis. Exp. (Pending Publication), e200140, doi:10.3791/200140 (2023).

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

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