JoVE Journal > Bioengineering

Preparation and Cell Seeding of Decellularized Apple-Derived Scaffolds

Published: February 23, 2024

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Maxime Leblanc Latour, Maryam Tarar, Ryan J. Hickey, Charles M. Cuerrier, Isabelle Catelas^4,5, Andrew E. Pelling^2,6,7

¹Department of Physics,University of Ottawa, ²Department of Biology,University of Ottawa, ³Department of Mechanical Engineering,University of Ottawa, ⁴Department of Surgery,University of Ottawa, ⁵Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ⁶Institute for Science, Society and Policy,University of Ottawa, ⁷SymbioticA, School of Human Sciences,University of Western Australia

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Keywords: Decellularized Apple-derived Scaffolds Mandolin Slicer 0.1% SDS Calcium Chloride Ethanol MC3T3-E1 Subclone 4 Cells Alpha MEM Fetal Bovine Serum Penicillin Streptomycin Trypsinization Cell Suspension Cell Seeding Differentiation Medium Ascorbic Acid Sodium Phosphate

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Preparation and Cell Seeding of Decellularized Apple-Derived Scaffolds. J. Vis. Exp. (Pending Publication), e200423, doi:10.3791/200423 (2024).

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