Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection

doi:10.3791/200471

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Biologia

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

Published: June 02, 2023

doi:

10.3791/200471

Emily Jie-Ning Yang, Istvan R. Boldogh², Haojie Ji, Liza Pon², Theresa C. Swayne²

¹Department of Pathology and Cell Biology,Columbia University Irving Medical Center, ²Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center,Columbia University Irving Medical Center

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Keywords: Yeast Cells Mitochondria HyPer7 Microscopy Imaging Fluorescence Confocal Microscopy SCMOS Camera Binning Laser Power Exposure Dynamic Range Noise Level Time-lapse Z-stacks Transmitted Light

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Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection. J. Vis. Exp. (Pending Publication), e200471, doi:10.3791/200471 (2023).

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

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