Peak Calling and Annotation in SCRAP for Small Noncoding RNA: Target RNA Interaction Studies

doi:10.3791/200890

JoVE Journal > Biology

Biologia

Peak Calling and Annotation in SCRAP for Small Noncoding RNA: Target RNA Interaction Studies

Published: December 01, 2023

doi:

10.3791/200890

Sreenivas Eadara, Xinbei Li, Emily A. Eiss, Mollie K. Meffert²

¹Department of Biological Chemistry,Johns Hopkins University School of Medicine, ²Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

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Peak_calling_directory=/path/to/sample/foldersAdapter_file=/path/to/adapter.fastaMin_reads=10Min_samples=2Require_same_family=TrueReference_dir=/path/to/reference/directoryMirbase_abbr=hsaGenome_abbr=hg38Peak_file=/path/to/resulting/peaks.bedBam_file=/path/to/merged.bamSorted_bam=/path/to/sorted.bamIndexed_bam=/path/to/sorted.bam.bai

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Peak Calling and Annotation in SCRAP for Small Noncoding RNA: Target RNA Interaction Studies. J. Vis. Exp. (Pending Publication), e200890, doi:10.3791/200890 (2023).

Peak Calling and Annotation in SCRAP for Small Noncoding RNA: Target RNA Interaction Studies

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