Quantification of Autoantibodies in the Serum of Experimental Autoimmune Encephalomyelitis Immunized Mice Using ELISA

JoVE Journal > Immunology and Infection

Imunologia e Infecção

Quantification of Autoantibodies in the Serum of Experimental Autoimmune Encephalomyelitis Immunized Mice Using ELISA

Published: December 01, 2023

doi:

10.3791/201206

Jonathan J. Carver, Alessandro Didonna

¹Department of Anatomy and Cell Biology, Brody School of Medicine,East Carolina University

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Keywords: ELISA Autoantibodies Serum Experimental Autoimmune Encephalomyelitis MOG 35-55 Peptide BSA Coating Buffer Blocking Solution HRP-conjugated Secondary Antibody TMB Substrate Optical Density

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Quantification of Autoantibodies in the Serum of Experimental Autoimmune Encephalomyelitis Immunized Mice Using ELISA. J. Vis. Exp. (Pending Publication), e201206, doi:10.3791/201206 (2023).

Quantification of Autoantibodies in the Serum of Experimental Autoimmune Encephalomyelitis Immunized Mice Using ELISA

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