Preparation of Anaerobic Liquid Medium in a Carboy for Culturing Environmental Anaerobic Microorganisms in a Bioreactor

Published: January 12, 2024

doi:

10.3791/201269

Tia Zimmerman, Garion Leamon, Georgia Dillenburg, Bethany Egge, Jennifer Pierce, Ben Elliott, Trevor Murphy, Marjorie Brooks, Scott D. Hamilton-Brehm

¹Department of Microbiology,Southern Illinois University Carbondale, ²MEDPREP,Southern Illinois University School of Medicine, ³Department of Geology,Southern Illinois University Carbondale, ⁴Department of Zoology,Southern Illinois University Carbondale

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Keywords: Anaerobic Liquid Medium Carboy Anaerobic Microorganisms Bioreactor Hose Barb Silicone Tubing Adapter Fitting Media Components PH Nitrogen Gas Sodium Bicarbonate Sodium Sulfide Nonahydrate Resazurin

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Preparation of Anaerobic Liquid Medium in a Carboy for Culturing Environmental Anaerobic Microorganisms in a Bioreactor. J. Vis. Exp. (Pending Publication), e201269, doi:10.3791/201269 (2024).

Preparation of Anaerobic Liquid Medium in a Carboy for Culturing Environmental Anaerobic Microorganisms in a Bioreactor

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