The mouse small bowel transplantation model has been recognized as an important tool to study mechanismes of immune rejection and screen new immunosuppressive drugs. However, this model is limited to use because the techniques involved is an extremely technically challenge. Now we introduce the modified technique.
Donor preparation and small bowel harvest:
Recipient preparation and transplantation:
The unique immune response after small bowel transplantation has been the subject of extensive research using small bowel transplantation model in mice. The basic immunological reactions, such as graft-versus-reactions, host-versus-graft-reaction, a combination of both reactions, chronic rejection and tolerance have been involved. Almost all immunosuppressive agents of proven or potential clinical relevance have been tested for their efficacy in small bowel transplantation model.
We modified the mouse small bowel transplant technique in several ways to improve efficiency and success rates:
Material Name | Tipo | Company | Catalogue Number | Comment |
---|---|---|---|---|
Sutures | 10-0 and 11-0 | MONOSOF | ||
Microscope | Unversal S3 | Carl Zeiss Inc |