Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

January 12, 2024

Anvendelse af MicroSiM (μSiM) barrierevævsplatformen til modellering af blod-hjerne-barrieren

µSiM Device Assembly for Blood Brain Barrier Monoculture

Etablering af blodhjernebarrieremonokultur i μSiM-enhed til immuncytokemisk farvning og konfokal analyse

Molly C. McCloskey, Pelin Kasap, Michelle Trempel, Louis P. Widom, Julia Kuebel, Kaihua Chen, Thomas R. Gaborski, Britta Engelhardt, James L. McGrath

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

Summary

Denne rapport indeholder protokoller til samling, cellekultur og analyser på μSiM-platformen til konstruktion af blod-hjerne-barrieremodeller.

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