Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Stephanie Ibrahim; Catherine Robert; Camille Humbert; Clotilde Ferreira; Gwena&#235;lle Collod; Sonia Stefanovic

doi:10.3791/65328

Nuclei Isolation from Mouse Cardiac Progenitor Cells at Single-Cell Resolution

May 12, 2023

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution (Video) | JoVE

01:46 min

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Mouse Cardiac Progenitor Cell Isolation, Digestion, and Freezing (Video) | JoVE

02:43 min

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Embryonic Heart Cell Thawing and Removal of Dead Cells (Video) | JoVE

04:22 min

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Nuclei Isolation from Cardiac Progenitor Cells (Video) | JoVE

01:45 min

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Stephanie Ibrahim, Catherine Robert, Camille Humbert, Clotilde Ferreira, Gwenaëlle Collod, Sonia Stefanovic

¹Center for Cardiovascular Disease & Nutrition, U1263,Aix-Marseille University, ²Marseille Medical Genetics, U1251,Aix-Marseille University

Summary

Here, we present a protocol describing cell nuclei preparation. After microdissection and enzymatic dissociation of cardiac tissue into single cells, the progenitor cells were frozen, followed by isolation of pure viable cells, which were used for single-nucleus RNA sequencing and the single-nucleus assay for transposase-accessible chromatin with high-throughput sequencing analyses.