Methods for Electroporation and Transformation Confirmation in <em>Limosilactobacillus reuteri</em> DSM20016

Alexander Duggan; David McMillen

doi:10.3791/65463

Measurement of Reporter Proteins in Limosilactobacillus reuteri DSM20016

June 23, 2023

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016 (Video) | JoVE

02:15 min

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Preparing Limosilactobacillus reuteri DSM20016 Electrocompetent Cells for Electroporation (Video) | JoVE

03:28 min

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Measurement of the Acid-Resistant Fluorescent Reporter Protein mCherry2 (Video) | JoVE

01:42 min

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Confirmation of Plasmid Uptake via Colony PCR (Video) | JoVE

01:48 min

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Mini-Prep Protocol for Limosilactobacillus reuteri and Confirmation of Plasmid Presence by PCR (Video) | JoVE

02:22 min

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Alexander Duggan, David McMillen

¹University of Toronto Mississauga

Summary

Here, we present protocols for working with Limosilactobacillus reuteri DSM20016, detailing growth, plasmid transformation, colony PCR, fluorescent reporter protein measurement, and limited plasmid mini-prep, as well as common issues and troubleshooting. These protocols allow the measurement of reporter proteins in DSM20016, or confirmation via colony PCR if no reporter is involved.