Live-Cell Imaging of <em>Drosophila melanogaster</em> Third Instar Larval Brains

Roberto Carlos Segura; Clemens Cabernard

doi:10.3791/65538

Imaging Live Brain Explants from Drosophila melanogaster Third Instar Larvae

June 23, 2023

Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants (Video) | JoVE

01:30 min

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Staging and Collecting Drosophila melanogaster Third Instar Larvae (Video) | JoVE

00:52 min

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Fat Body Collection from Drosophila melanogaster Third Instar Larvae (Video) | JoVE

00:55 min

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Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging (Video) | JoVE

01:41 min

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Mounting and Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brain (Video) | JoVE

02:38 min

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Roberto Carlos Segura, Clemens Cabernard

¹Department of Biology,University of Washington

Summary

Here, we discuss a workflow to prepare, dissect, mount, and image live explant brains from Drosophila melanogaster third instar larvae to observe the cellular and subcellular dynamics under physiological conditions.