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Reprogramming PDAC and Pancreatic Cells into Induced Pluripotent Stem Cells

February 2nd, 2024
1,2, 3, 4, 4,5, 4,6, 3,6, 1
1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, 5Department of Surgery, Oregon Health & Science University, 6Department of Molecular & Medical Genetics, Oregon Health & Science University

Summary
Automatic Translation

Het huidige protocol beschrijft de herprogrammering van ductaal adenocarcinoom van de pancreas (PDAC) en normale ductale epitheelcellen van de pancreas tot geïnduceerde pluripotente stamcellen (iPSC's). We bieden een geoptimaliseerde en gedetailleerde, stapsgewijze procedure, van het bereiden van lentivirus tot het opzetten van stabiele iPSC-lijnen.

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