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Reprogramming PDAC and Pancreatic Cells into Induced Pluripotent Stem Cells

February 2nd, 2024
1,2, 3, 4, 4,5, 4,6, 3,6, 1
1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, 5Department of Surgery, Oregon Health & Science University, 6Department of Molecular & Medical Genetics, Oregon Health & Science University

Summary
Automatic Translation

El presente protocolo describe la reprogramación del adenocarcinoma ductal pancreático (PDAC) y las células epiteliales ductales pancreáticas normales en células madre pluripotentes inducidas (iPSC). Proporcionamos un procedimiento optimizado y detallado, paso a paso, desde la preparación de lentivirus hasta el establecimiento de líneas estables de iPSC.

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