Multiplex RT-qPCR Assay for Respiratory Virus Detection

November 10, 2023

Advancements in Multiplex Detection of Respiratory Viruses (Video) | JoVE

01:10 min

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Preparation and Testing of Multiplex R3T One-Step RT-qPCR Kit Components (Video) | JoVE

02:43 min

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Atheer Althobaiti, Kareem Hamdan, Mohamed A. Sobhy, Renad Rawas, Masateru Takahashi, Olga Artyukh, Muhammad Tehseen

¹Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Summary

We present two probe-based in-house one-step RT-qPCR kits for common respiratory viruses. The first assay is for SARS-CoV-2 (N), Influenza A (H1N1 and H3N2), and Influenza B. The second is for SARS-Cov-2 (N) and MERS (UpE and ORF1a). These assays can be successfully implemented in any specialized laboratory.

Multiplex RT-qPCR Assay for Respiratory Virus Detection

Advancements in Multiplex Detection of Respiratory Viruses (Video) | JoVE

Preparation and Testing of Multiplex R3T One-Step RT-qPCR Kit Components (Video) | JoVE

Summary

Article