Microdissection and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins

Hannes E. Villgrater; Ruibing Xia; Aparna Sharma Chivukula; Philipp Tomsits; Sebastian Clauss

doi:10.3791/65836

Revealing Murine Pulmonary Veins Myocardial Architecture

November 21, 2023

Developing a Translational Model for Atrial Fibrillation Research Across Species (Video) | JoVE

01:03 min

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Microdissection Protocol for Isolating the Murine Left Atrium (LA) with the Pulmonary Veins (Video) | JoVE

03:42 min

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Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins (Video) | JoVE

04:14 min

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Hannes E. Villgrater*^1,2,3, Ruibing Xia*^1,2,3, Aparna Sharma Chivukula^2,3, Philipp Tomsits*^1,2,3, Sebastian Clauss*^1,2,3,4

¹University Hospital Munich, Department of Medicine I,Ludwig-Maximilians-University Munich (LMU), ²Walter Brendel Center of Experimental Medicine (WBex),LMU Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ⁴Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU),LMU Munich

Summary

This protocol demonstrates microscopy-guided isolation and immunofluorescence staining of murine pulmonary veins. We prepare tissue samples containing the left atrium, pulmonary veins, and the corresponding lungs and stain them for cardiac Troponin T and Connexin 43.

Revealing Murine Pulmonary Veins Myocardial Architecture

Developing a Translational Model for Atrial Fibrillation Research Across Species (Video) | JoVE

Microdissection Protocol for Isolating the Murine Left Atrium (LA) with the Pulmonary Veins (Video) | JoVE

Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins (Video) | JoVE

Summary

Article