1Orthopaedic Stem Cell Research Laboratory,Cedars-Sinai Medical Center, 2Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 3Department of Biomedical Sciences,Cedars-Sinai Medical Center, 4Department of Orthopedics,Cedars-Sinai Medical Center, 5Department of Surgery,Cedars-Sinai Medical Center
This article describes a procedure to produce iTenocytes by generating iPSC-derived mesenchymal stromal cells with combined overexpression of Scleraxis using a lentiviral vector and uniaxial stretching via a 2D bioreactor.
