Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models

February 02, 2024

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Rosa L. Vincent, Fangda Li, Edward R. Ballister, Nicholas Arpaia³, Tal Danino^3,4

¹Department of Biomedical Engineering,Columbia University, ²Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, ³Herbert Irving Comprehensive Cancer Center,Columbia University, ⁴Data Science Institute,Columbia University

Summary

This protocol streamlines retroviral vector production and murine T cell transduction, facilitating the efficient generation of mouse CAR-T cells.

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