Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models

February 02, 2024

Effektiv generering af murine kimære antigenreceptorceller (CAR)-T-celler

Transfektion og transduktion af murin T-celler til CAR-T-cellegenerering

Rosa L. Vincent, Fangda Li, Edward R. Ballister, Nicholas Arpaia³, Tal Danino^3,4

¹Department of Biomedical Engineering,Columbia University, ²Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, ³Herbert Irving Comprehensive Cancer Center,Columbia University, ⁴Data Science Institute,Columbia University

Summary

Denne protokol strømliner retroviral vektorproduktion og murine T-celletransduktion, hvilket letter effektiv generering af muse-CAR-T-celler.

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