Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

活小鼠精子顶体钙动力学和胞吐作用的实时成像

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging

Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm

Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

Roy Cohen², Danielle M. Sosnicki, Melissa A. White, Jacquelyn L. Nelson, Chinatsu Mukai, Alexander J. Travis²

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Summary

这里描述的AcroSensE小鼠模型和活细胞成像方法为研究精子顶体亚细胞区室中的钙动力学以及它们如何调节导致膜融合和顶体胞吐作用的中间步骤提供了一种新方法。

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