Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Roy Cohen; Danielle M. Sosnicki; Melissa A. White; Jacquelyn L. Nelson; Chinatsu Mukai; Alexander J. Travis

doi:10.3791/65962

Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

Imagem em Tempo Real da Dinâmica do Cálcio Acrossomal e Exocitose em Espermatozoides Vivos de Camundongos

00:46 min

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Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging

01:27 min

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Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm

01:19 min

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Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

01:45 min

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Roy Cohen², Danielle M. Sosnicki, Melissa A. White, Jacquelyn L. Nelson, Chinatsu Mukai, Alexander J. Travis²

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Summary

O modelo de camundongo AcroSensE e os métodos de imagem de células vivas descritos aqui fornecem uma nova abordagem para estudar a dinâmica do cálcio no compartimento subcelular do acrossoma espermático e como eles regulam etapas intermediárias que levam à fusão da membrana e exocitose acrossomal.