Live Tracking of Dental Cells via Two-Photon Microscopy

October 27, 2023

使用离体实时成像研究小鼠牙齿更新过程中的细胞分裂和运动

Dissection and Isolation of Mouse Mandibular Incisors and Associated Periodontal Tissues to Expose the Incisor Epithelial Cervical Loop

Periodontal Tissue Embedding and Timelapse Microscopy of Mouse Incisor Explant Cultures

Abinaya Sundari Thooyamani*¹, Elias Shahin*², Sanako Takano, Amnon Sharir, Jimmy K. Hu³

¹School of Dentistry,University of California Los Angeles, ²The Institute of Biomedical and Oral Research, Faculty of Dental Medicine,Hebrew University of Jerusalem, ³Molecular Biology Institute,University of California Los Angeles

Summary

离体实时成像是一种强大的技术，用于研究活组织中细胞运动和相互作用的动态过程。在这里，我们提出了一种方案，该方案实施双光子显微镜以实时跟踪培养的全成年小鼠门牙中的牙齿上皮细胞。

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