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Ultracentrifuge-Based Isolation of Extracellular Vesicles from Human ADSCs

May 03, 2024
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Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

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1Department of Plastic and Reconstructive Surgery, the First Medical Centre,Chinese PLA General Hospital, 2School of Medicine,Nankai University

Summary

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This protocol describes all procedures, from culturing human adipose-derived mesenchymal stem cells (ADSCs) and collecting supernatant to extracting extracellular vesicles (EVs) using ultracentrifugation.

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