1Translational Research Institute,AdventHealth, 2Steno Diabetes Center Copenhagen, 3Division of Endocrinology and Metabolism,University of Pittsburgh, 4Gerontology and Geriatric Medicine,Wake Forest University School of Medicine, 5Department of Internal Medicine – Rheumatology, Allergy, and Clinical Immunology,University of California Davis Health
The biology of intermuscular adipose tissue (IMAT) is largely unexplored due to the limited accessibility of human tissue. Here, we present a detailed protocol for nuclei isolation and library preparation of frozen human IMAT for single nuclei RNA sequencing to identify the cellular composition of this unique adipose depot.
