JOVE JOURNAL

Bioquímica

Showing 433 - 444 in 724 videos.

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)

Daniel Some, Hadar Amartely, Ayala Tsadok, Mario Lebendiker

¹Wyatt Technology Corporation, ²Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, ³Danyel Biotech Ltd.

Journal (Biochemistry)

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Shan Feng², Xiaofei Wen, Xin Lu^4,5,6

¹Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, ²Department of Biological Sciences,University of Notre Dame, ³Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, ⁴Boler-Parseghian Center for Rare and Neglected Diseases,University of Notre Dame, ⁵Harper Cancer Research Institute,University of Notre Dame, ⁶Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center

Journal (Biochemistry)

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

Weiting Jiao*^1,2, Guoqin Ge*¹, Rimao Hua, Jun Sun, Yeyun Li, Ruyan Hou

¹State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology,Anhui Province Key Lab of Analysis and Detection for Food Safety, ²School of Resource & Environment, Anhui Agricultural University,Key Laboratory of Agri-food Safety of Anhui Province

Journal (Biochemistry)

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

Vinciane Saint-Criq, Iram J. Haq³, Aaron I. Gardner, James P. Garnett⁴, Christopher Ward², Malcolm Brodlie³, Michael A. Gray

¹Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, ²Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, ³Paediatric Respiratory Medicine, Great North Children's Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, ⁴Boehringer Ingelheim Pharma GmbH & Co

Journal (Biochemistry)

Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

Cristina Mascaró, Raquel Ruiz Rodriguez, Tamara Maes

¹Oryzon Genomics

Journal (Biochemistry)

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Carlos Flores^2,3, Paula Tamagnini^2,4

¹Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude,Universidade do Porto, ²Instituto de Biologia Celular e Molecular,Universidade do Porto, ³Instituto de Ciências Biomédicas Abel Salazar, ⁴Departamento de Biologia, Faculdade de Ciências,Universidade do Porto

Journal (Biochemistry)

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Patrick Moran², Yichen Guo⁴, Rong Yuan³, Nicholas Barnekow, Jordan Palmer, Adam Beck, Bin Ren^4,5

¹Blood Research Institute,Blood Center of Wisconsin, ²Department of Medicine,Medical College of Wisconsin, ³Department of Surgery,The University of Alabama at Birmingham, ⁴Department of Biomedical Engineering,The University of Alabama at Birmingham, ⁵GBS Program, Graduate School,The University of Alabama at Birmingham

Journal (Biochemistry)

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Alexanne Cuillerier, Yan Burelle

¹Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine,University of Ottawa

Journal (Biochemistry)

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Shellaina J.V. Gordon*¹, Yao Xiao*², Amanda L. Paskavitz, Napoleón Navarro-Tito, Juan G. Navea, Teresita Padilla-Benavides

¹Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, ²Department of Chemistry,Skidmore College, ³Candiac MR Center,Beth Israel Deaconess Medical Center, ⁴Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Journal (Biochemistry)

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Phillip C. Delekta, Todd A. Lydic, Neal D. Hammer

¹Department of Microbiology and Molecular Genetics,Michigan State University, ²Department of Physiology,Michigan State University

Journal (Biochemistry)

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Chase M. Clark, Maria S. Costa², Erin Conley, Emma Li, Laura M. Sanchez, Brian T. Murphy

¹Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy,University of Illinois at Chicago, ²Faculty of Pharmaceutical Sciences,University of Iceland

Journal (Biochemistry)

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Markus Axmann, Andreas Karner, Sabine M. Meier, Herbert Stangl, Birgit Plochberger

¹Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, ²School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

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