JOVE JOURNAL

Bioquímica

Showing 649 - 660 in 731 videos.

Oligopeptide Competition Assay for Phosphorylation Site Determination

Min Sung Joo, Ja Hyun Koo, Sol-Bi Shin, Hyungshin Yim, Sang Geon Kim

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Journal (Biochemistry)

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Rachel A. Battaglia, Parijat Kabiraj, Helen H. Willcockson, Melinda Lian, Natasha T. Snider

¹Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Measuring Protein Binding to F-actin by Co-sedimentation

Jonathon A. Heier, Daniel J. Dickinson, Adam V. Kwiatkowski

¹Department of Cell Biology,University of Pittsburgh School of Medicine, ²Department of Biology,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Meong Il Kim, Choongdeok Lee, Minsun Hong

¹Division of Biological Science and Technology,Yonsei University

Journal (Biochemistry)

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Toshio Moriya, Michael Saur, Markus Stabrin, Felipe Merino, Horatiu Voicu, Zhong Huang, Pawel A. Penczek, Stefan Raunser, Christos Gatsogiannis

¹Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Journal (Biochemistry)

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Junyan Ma, Inna S. Yanez-Orozco, Soheila Rezaei Adariani, Drew Dolino, Vasanthi Jayaraman, Hugo Sanabria

¹Department of Chemistry,Clemson University, ²Department of Physics and Astronomy,Clemson University, ³Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences,University of Texas Health Science Center

Journal (Biochemistry)

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson, Saffron Little, Dana S. Franklin, Jennifer A. Gaddy³, Steven M. Damo⁴

¹Department of Life and Physical Sciences,Fisk University, ²Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases,Vanderbilt University Medical School, ⁴Department of Biochemistry,Vanderbilt University

Journal (Biochemistry)

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Xavier Morató*^1,2, Marc López-Cano*^1,2, Paula M. Canas, Rodrigo A. Cunha, Francisco Ciruela²

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L'Hospitalet de Llobregat, ²Institut de Neurociències,Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Journal (Biochemistry)

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Tyler Rhinesmith, Bryan A. Killinger, Akhil Sharma, Anna Moszczynska

¹Physiology,Michigan State University, ²Center for Neurodegenerative Science Van Andel Institute, ³Pharmaceutical Sciences,Wayne State University

Journal (Biochemistry)

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Melissa Bredow, Heather E. Tomalty, Virginia K. Walker^2,3

¹Department of Biology,Queen's University, ²Department of Biomedical and Molecular Sciences,Queen's University, ³School of Environmental Sciences,Queen's University

Journal (Biochemistry)

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Christina D. King, Joseph D. Dudenhoeffer, Liqing Gu, Adam R. Evans, Renã A. S. Robinson

¹Department of Chemistry,University of Pittsburgh, ²SGS North America Inc., ³Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Journal (Biochemistry)

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Tobias G. Kapp*¹, Maximilian Fottner*¹, Horst Kessler

¹Department of Chemistry,Technische Universität München

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